![]() burgdorferi blot).The screening test and/or blot for B. Positive or equivocal screening test results should not be interpreted as truly positive until verified as such using a supplemental assay (e.g., B. Immunoblot testing is appropriate for confirming a detected screening result.Īdditional Information: As recommended by the Food and Drug Administration (FDA), all samples with positive or equivocal results in a Borrelia burgdorferi antibody screen will be tested using a blot method. Methodology: Immunoassay (IA) Reflex: Immunoblot (IB)Ĭlinical Significance: Lyme Disease Antibody with Reflex to Blot (IgG, IgM) – Lyme disease is caused by a bacterium borrelia burgdorferi and is transmitted by ticks. A screening test with high sensitivity is used as the first step in the CDC recommended algorithm. Immunoblot testing qualitatively examines, with high specificity, antibodies in a patient’s specimen. Transport: Store serum at 2-8☌ after collection and ship the same day per packaging instructions included with the provided shipping box.Ĭauses for Rejection: Specimens other than serum improper labeling samples not stored properly samples older than stability limits grossly hemolyzed, grossly lipemic, or grossly icteric samples Let tube stand in a vertical position to allow blood to clot 30 minutes.Gently invert tube 5 times immediately after draw.Collect and label sample according to standard protocols.If Lyme Disease Ab Screen is ≥0.90, then Lyme Disease Ab (IgG), Blot and Lyme Disease Ab (IgM), Blot will be performed at an additional charge (CPT Codes: 86617 x2).Ĭontainer: Gel-barrier tube (SST, Tiger Top)
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